Trophoblast attachment to the endometrial epithelium elicits compartment-specific transcriptional waves in an in-vitro model

Research questionWhich are the early compartment-specific transcriptional responses of trophoblast and endometrial epithelium throughout attachment during implantation?DesignAn proxy (cell line Ishikawa) was co-cultured with spheroids a green fluorescent protein (GFP) expressing cell (JEG-3). After 0, 8 24 h co-culture, compartments were sorted by fluorescence-activated sorting; GFP+ (trophoblast), GFP– (epithelium) non-co-cultured control populations analysed (in triplicate) RNA-seq gene set enrichment analysis (GSEA).ResultsTrophoblast challenge induced wave changes in that resulted 295 differentially regulated genes involving epithelial to mesenchymal transition (EMT), movement, apoptosis, hypoxia, inflammation, allograft rejection, myogenesis signalling at h. Interestingly, many enriched pathways subsequently de-enriched (i.e. EMT, signalling). In trophoblast, co-culture more regulation variety pathways. A total 1247 481 expressed after from h, respectively. Angiogenesis hypoxia over-represented both stages, while EMT only h; inflammation oestrogen response enriched, proliferation under-represented.ConclusionsSuccessful produced series dynamic expression, characterized an overall transient up-regulation receptive epithelium, contrast trophoblast.